Acetaminophen Toxicity in Children: Diagnosis, Clinical Assessment, and Treatment of Acute Overingestion Consultant360 Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine The Hidden Impact of Acetaminophen Well Rooted Is Tylenol Safe During Pregnancy? Risk Factors, Not Causation Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.