Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Serotonin Deficiency Exacerbates Acetaminophen Induced Liver Toxicity In Mice Scientific Reports Acetaminophen metabolism Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports